Since there has been a bit of discussion of focus stacking recently, I thought it might be helpful to post an example, pointing out some of the issues that have been raised.
First, here is the final image of an anemone:
I shot this last night with my 50D and a 100mm macro. All shots were f/7.1. Lighting was two halogen floods in hair lights, one with a diffuser and one using an umbrella. There were 11 images, which I stacked using Zerene. I used the DMap algorithm, which is slower than PMax and supposedly preserves a bit less detail, because I have found that DMAP does much better at preserving colors and textures. It has a bit of editing in Lightroom after stacking.
The following two images are the first and last in the stack:
You can see that even at f/7.1 (at close to minimum working distance), DOF covers very little of the image, particularly because it is a deep subject, and I have it deliberately placed not parallel to the sensor. You'll see that the last image, which I needed to have the back anthers in focus, also has the petals in focus, which I did not want (Glenn's point in an earlier post).
BTW, I always start by framing the subject with the camera focused at the closest position needed. that is because the image gets smaller as you focus closer. If you frame starting at the back, you will lose material from the edges as you move in from shot to shot.
Now, here is what I think is the interesting part, the initial stacked image:
This image has two problems. First, I think it is harsh with the petals in focus. Second, there is a small amount of haloing from parallax. I mentioned in an earlier post that Zerene never gives me the streaking from parallax that I had with one other program. However, it does sometimes create haloing around edges if the surface behind the edge is too far back. If you look at this larger image:
http://dkoretz.smugmug.com/photos/i-...PDP9Xqz-X3.jpg
You can see a bit of haloing around some of the anthers.
The solution to both problems is Zerene's retouching tool, which acts somewhat like the brush tool in photoshop. Zerene will show you any of the source images on one side of the screen and the stacked image in the other. You can then paint from the original to the stacked image. So, I first replaced the petals in the stacked image with the petals from the front-most image above. I then zoomed in and selected other images from the stack, as needed, to do a bit better cleanup of some of the haloing.
I hope this is helpful. And, no, I have no connection to Zerene, other than being a user.
Dan