Spurred by this, I just did some controlled tests with my Canon 5DIII (a fairly low-noise camera), shooting the exact same scene with ISOs from 100 to 6400. The results surprised me. That's one nice thing about empirical data.
I suggest looking at all of these in the lytebox.
One thing that is relevant is that the lighting was ideal, extending almost the full range, and with the majority of mass in the top half of the histogram:
https://dkoretz.smugmug.com/photos/i.../i-qdj5gcz.jpg
I deliberately chose a scene with a flat surface, to show noise, and some detail, to show the impact of noise on sharpness. For the latter, look at the lettering on the red and white White Mountains guide, which was the point of focus.
I shot these at f/7.1 using a macro lens. I did no processing at all other than reading them into lightroom and allowing lightroom to apply its default capture sharpening and color noise reduction. I exported the jpegs at 92% quality with output sharpening turned off.
ISO 100:
https://dkoretz.smugmug.com/photos/i...6Wc956p-XL.jpg
ISO 800:
https://dkoretz.smugmug.com/photos/i...NW2cG9J-XL.jpg
ISO 6400:
https://dkoretz.smugmug.com/photos/i...2V6mbxq-XL.jpg
At 100%, the ISO 6400 image shows degradation, although less than I thought. You can see the full-size jpeg here:
https://dkoretz.smugmug.com/photos/i.../i-2V6mbxq.jpg
Some observations:
-- As expected, at this level of downsampling, they all look fine. The effects, when present, will be more apparent at larger sizes.
-- At 100%, the degradation of the image is considerably less than I had expected given past experience. For example, here is a 100% crop from an ISO 4000 image, with the ISO selected by the camera, after processing but without noise reduction other than LR's default:
https://dkoretz.smugmug.com/photos/i.../i-rPpb9qm.jpg
This one looks fine when downsampled to a small online display, but the image degradation is substantial.
I think the reason is apparent from the histogram, which is very different from the first:
https://dkoretz.smugmug.com/photos/i.../i-zn5vfXm.jpg
So, my take-aways:
1. Don't use small online displays to evaluate image quality, unless that is the only way you are going to present the image. They will obscure problems that may be apparent at larger sizes.
2. Modern lower-noise cameras can handle fairly high ISOs reasonable well, if the lighting is pretty good, meaning that the distribution of luminance has its mass fairly far toward the bright end.
3. If you have to use high ISOs with images that have a lot of the mass at the darker end, the results will not be as good.
Seem like reasonable conclusions?
Dan